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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Direct inhibition of ACTN4 by ellagic acid limits breast cancer metastasis via regulation of β-catenin stabilization in cancer stem cells

Fig. 6

ACTN4 promotes breast cancer proliferation and metastasis in vitro and in vivo. a Intracellular expression of ACTN4 was determined by Western blot (left) and real-time PCR (right) analysis, respectively; b ACTN4 expression was modified by transfecting recombinant plasmid or its shRNA in breast cancer cells and subjected to Western blotting (left) and real-time PCR (right) validation; c MTT and colony formation assay showed that ACTN4 silencing abrogated breast cancer cell proliferation while its overexpression promoted cell growth; d ACTN4 silencing inhibited the migration and invasion abilities of MDA-MB-231 cells; e ACTN4 silencing inhibited breast cancer growth and lung metastasis in vivo (*P < 0.05, **P < 0.01 versus control, values represented as the mean ± SD, n = 3)

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