Fig. 1

ID1 is overexpressed in oxaliplaitin-resistant HCC cell lines and involved in HCC cell proliferation, apoptosis and chemoresistance in vitro. a ID1 expression was analyzed by Western blot and Real-time RT-PCR assays in oxaliplaitin-resistant HCC cell lines. b 97H–OXA and 3B–OXA cells were infected by lentiviral vectors with short hairpin RNA clone targeting ID1 gene. After transfection, ID1 knockdown was confirmed by western blot analysis and Real-time RT-PCR assays. c 97H–OXA-Ctrol, 97H–OXA-shID1, 3B–OXA-Ctrol and 3B–OXA-shID1 cells were subjected to colony formation ability assay. d CCK-8 assay showed that the proliferation of 97H–OXA-shID1 and 3B–OXA-shID1 cells was inhibited upon silencing ID1 expression (* P < 0.05). The data are indicated as means of three independent assays. e Cell apoptosis was determined by flow cytometry using Annexin V FITC apoptosis detection kit. The number of apoptotic 97H–OXA-shID1 and 3B–OXA-shID1 cells was significantly increased upon silencing ID1 expression (P = 0.001 and P < 0.001). f After treatment with different concentrations of oxaliplaitin for 48 h, the chemoresistance of HCC cells was evaluated by CCK-8 assay. The IC50 value in 97H–OXA or 3B–OXA cells was significantly higher than that in 97H–OXA-shID1 or 3B–OXA-shID1 cells (P = 0.001 and P < 0.001, respectively)