Fig. 2

Silencing ID1 expression inhibits HCC cell proliferation, apoptosis and chemoresistance in vivo. a 97H–OXA-Ctrol and 97H–OXA-shID1 cells were injected subcutaneously into the right flank of nude mice. One week after implantation, mice were treated with 0.1 ml oxaliplatin (10 mg/kg/week) via tail vein injection for 4 weeks. b The graph of tumor kinetics showed that ID1 knockdown inhibited tumor growth in 97H–OXA-shID1 group treated with oxaliplatin (* P < 0.05) (c) 6 weeks after implantation, 97H–OXA-Ctrol cells produced larger tumors than 97H–OXA-shID1 cells (P = 0.002). The data are presented as the mean ± SD of five tumors per group. d and e The apoptosis rate in 97H–OXA-shID1 cells was higher than that in 97H–OXA-Ctrol cells as determined by TUNEL analysis (P = 0.002). f IHC analysis of the apoptosis-related and proliferation-related protein expression in 97H–OXA-Ctrol tumor and 97H–OXA-shID1 tumor. ID1 knockdown increased the expression of Caspase-3 and Caspase-9 and inhibited the expression of Ki-67