Fig. 6

ID1 modulates c-MYC activation through Wnt/β-catenin pathway. a and b c-MYC expression was down-regulated upon ID1 knockdown, as indicated in Western blot and Real-time RT-PCR assays. c Direct interaction of ID1 and c-MYC protein was explored by coimmunoprecipitation. Protein was immunoprecipitated (IP) with anti-ID1 antibody and was immunoblotted to detect c-MYC expression. Normal mouse IgG was used as a control antibody. d G6PD promoter transcription activity was increased upon the pGL3-ID1 plasmid concentration increasing from 0.5 to 2μg. e Increased c-MYC expression from 0.5 to 2μg was detected in HEK293T cells transfected with pGL3-ID1 plasmid. f Western Blot showed that Wnt/β-catenin pathway, rather than MAPK/ERK pathway was regulated by ID1expression. g and h ID1 induced c-MYC and G6PD expression was inhibited by FH535, a specific small-molecule inhibitor of Wnt/β-catenin pathway (10umol/L for 48H)