Fig. 3
From: Adipocytes promote cholangiocarcinoma metastasis through fatty acid binding protein 4
Adipose tissue extracts facilitate CCA metastasis in nude mice. RBE cells (1 × 106) were separately incubated in growth medium, adipose extracts medium, and adipose extracts plus fatty acid uptake inhibitor BMS309403, and then injected through the tail vein of Balb/C nude mice to construct the CCA metastasis mice model (a). 24 h after inoculation, the in vivo migration and invasion were observed using the living animal imaging system (b), and were quantified by fluorescence intensity (c). One month after injection, mice were euthanized. Histopathological analysis of livers and lungs were performed on formaldehyde fixed-paraffin embedded sections via H&E staining (d). n = 5 per group, *P < 0.05; a P < 0.01