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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Adipocytes promote cholangiocarcinoma metastasis through fatty acid binding protein 4

Fig. 5

Inhibition of FABP4 suppresses the metastasis of RBE cells. Parental RBE cell and RBE treated with adipose extracts or adipose extracts plus 20 μM/L BMS309403 for 24 h were utilized for the following analysis. The immunofluorescence staining of intracellular lipids was determined using fluorescence dye Bodipy under the confocal microscopy (a). The invasiveness and migration of RBE cells with different culture condition was determined via transwell invasion assay (b) and wound healing assay (c) as described above. The EMT related protein expression was detected via Western blot assay (d). All the samples were prepared in triplicate, and all experiments were repeated at least three times. *P < 0.05

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