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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Adipocytes promote cholangiocarcinoma metastasis through fatty acid binding protein 4

Fig. 7

FABP4 overexpression enhances the in vitro metastasis of RBE cells. RBE cells were infected with FABP4 recombinant adenovirus (1:100) and control adenovirus for 24 h, and then were incubated in adipose extracts for additional 24 h. To measure the intracellular lipids, Bodipy immunofluorescence staining was performed as described above (a). The in vitro invasion (b) and migration (c) of Hccc-9810 were analyzed and quantified as described above. Western blot assay was performed to detect the expression levels of EMT related protein (d). All the samples were prepared in triplicate, and all experiments were repeated at least three times. *P < 0.05

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