Fig. 2

ALX4 suppresses breast cancer cell proliferation and metastasis in vitro. a Transfectants of the control vector and ALX4 were identified WB and RT-PCR in MDA-MB-231 and MCF-7 cell lines. b MTS assays were performed to examine the effect of ALX4 over expression on the relative number of viable cells based on the absorbance. c Over expression of ALX4 reduced the colony formation ability of MDA-MB-231 and MCF-7 cell lines. Error bars indicate s.d. (n = 3).* P < 0.05, ** P < 0.01. d EDU assay was performed to detect the effect of ALX4 over expression on proliferation. e Cell cycles were analyzed after ALX4 over expression using PI stainning. f Transwell assays were used to examine the effect of ALX4 on cell migration and invasion in MDA-MB-231 and MCF-7 cell lines