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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: ALX4, an epigenetically down regulated tumor suppressor, inhibits breast cancer progression by interfering Wnt/β-catenin pathway

Fig. 4

ALX4 inhibits tumor formation and metastasis in nude mice. a Control vector and ALX4 stably expressing MDA-MB-231 cells (8 × 106) were subcutaneously injected into the right flank of nude mice. Pictures of BALB/c-nude mice and solid tumor tissues were taken after 33 days. b The tumor growth curve of ALX4 over expressing cells was compared with vector control cells. c Tumor weights in the vector control and ALX4 groups were determined. Error bars indicate s.d. (n = 3). d Liver metastases were observed by H&E staining in the control group but not in the ALX4 group by tail veins injection method. Arrows indicate the metastatic loci. e Liver metastasis was further quantified using RT-qPCR. Human-specific β2-MG levels were used to quantify metastatic human cancer cells with the mouse-specific β2-MG level as an internal control. The nude mice that were injected with ALX4 over expressing cells demonstrated a significantly lower number of metastatic cancer cells in the liver compared with those that were injected with an empty control. Error bars indicate s.d. (n = 3).*P < 0.05; **P < 0.01

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