Skip to main content
Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: ALX4, an epigenetically down regulated tumor suppressor, inhibits breast cancer progression by interfering Wnt/β-catenin pathway

Fig. 5

ALX4 suppresses the Wnt/β-catenin pathway. a ALX4 was positively correlated with CTNNBIP1 expression (R = 0.093, P = 0.00) and negatively correlated with Cyclin D1 expression (R = −0.102, P = 0.00). b ALX4 decreased the transcriptional activity of the Wnt/β-catenin pathway as determined by a TOP flash/FOP flash reporter luciferase activity assay in MDA-MB-231 and MCF-7 cell lines. TOP and FOP flash plasmids were co-transfected with control vector or ALX4 and PRL-TK plasmids in MDA-MB-231 and MCF-7 cells. c, d ALX4 inhibited the expression of Wnt/β-catenin pathway target genes at both the mRNA and protein levels. Error bars indicate s.d. (n = 3). *P < 0.05; **P < 0.01

Back to article page