Fig. 6

ALX4 represses the Wnt/β-catenin pathway by promoting the phosphorylation degradation of β-catenin. a The full length human β-catenin promoter (−2760 bp to +27 bp) was cloned into the PGL3-Basic luciferase reporter vector. The β-catenin transcription activity was examined after ectopic expression of ALX4 in MDA-MB-231 and MCF-7 cell lines. b ALX4 promoted the phosphorylation degradation of β-catenin in breast cancer cell lines. c Knock down of GSK3β by siRNA in MDA-MB-231-ALX4 cells decreased the p-β-catenin and recovered the protein level of β-catenin. d The protein expression of β-catenin, p-β-catenin and GSK3β were detected in xenograft tumors