Fig. 1
From: Autophagy maintains the stemness of ovarian cancer stem cells by FOXA2.
OCSCs were enriched from 3AO and SKOV3 ovarian cancer cells. (a)Spheres derived from 3AO and SKOV3 ovarian cancer cells maintained in serum-free medium culture system at 6 days (×200). (b) FCM (Flow Cytometry) analysis of CD24 and CD44 expression in 3AO and SKOV3 parental cells and sphere cells. (c) Protein expression of stemness regulators NES, NANOG, POU5F1 in 3AO and SKOV3 parental and sphere cells. (d) Messenger RNA expression of stemness regulators NES, NANOG, POU5F1 in 3AO, SKOV3 parental and sphere cells. Three independent experiments were performed and the results were expressed as the means ± SD, and analyzed using Student’s t-test (* P < 0.05, **P < 0.01)