Fig. 5

FOXA2 is required for self-renewal ability of OCSCs. 3AO and SKOV3 spheres were transfected with lentivirus expressing shRNA for FOXA2 (shFOXA2) or a scrambled shRNA (Ctrl). (a) Cells were seeded at 200 cells per well in a 96-well ultralow attach plates and grown for 6 days, and monitored under a microscope (upper); the size and number of the spheres were quantified (bottom). (b) The CD24⁻ phenotype of 3AO spheres (left) and the CD44⁺ phenotype of SKOV3 spheres (right) were determined by FCM. Three independent experiments were performed and data are shown asmeans ± SD(* P < 0.05, **P < 0.01)