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Fig.6 | Journal of Experimental & Clinical Cancer Research

Fig.6

From: Autophagy maintains the stemness of ovarian cancer stem cells by FOXA2.

Fig.6

.Autophagy modulates the self-renewal of OCSCs through FOXA2 participation. (a) 3AO and SKOV3 cells were transfected with pEGFP(+)-FOXA2(+) or pEGFP(+)(Ctrl)plasmid and the FOXA2expression levels were assessed by Western Blotting and qRT-PCR. (b) The indicated cells were seeded at 200 cells per well in a 96-well ultralow attach plates and grown for 6 days, and were then treated with BafA1 (10 nM, 48 h) or not, and monitored under a microscope (left). The size and the number of the spheres were quantified (right). (c) Cells were treated and cultured as above; the CD24 phenotype of 3AO spheres (upper) and the CD44+ phenotype of SKOV3 spheres (bottom) were determined by FCM. Three independent experiments were performed and the results were expressed as the means ± SD, and analyzed using Student’s t-test (* P < 0.05, **P < 0.01)

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