Fig. 1

miR-141-3p is downregulated in bone metastatic PCa tissues and cells. a miR-141-3p expression levels was decreased in bone metastatic PCa tissues (PCa/BM) compared with that in non-bone metastatic PCa tissues (PCa/nBM) as assessed by analyzing the TCGA PCa miRNA sequencing dataset (PCa/nBM, n = 11; PCa/BM, n = 9). b Percentages and number of samples showed high or low miR-141-3p expression in bone metastatic and non-bone metastatic PCa tissues in PCa dataset from TCGA. c Real-time PCR analysis of miR-141-3p expression in 89 non-bone metastatic and 52 bone metastatic PCa samples. Transcript levels were normalized to U6 expression. d Percentages and number of samples showed high or low miR-141-3p expression in bone metastatic and non-bone metastatic PCa tissues in our PCa tissues. e Real-time PCR analysis of miR-141-3p expression levels in normal prostate epithelial cell (RWPE-1), primary PCa cell 22RV1, bone metastatic PCa cell lines (PC-3, C4-2B and VCaP) and brain metastatic cell line DU145 and lymph node metastatic cell line LNCaP. Transcript levels were normalized to U6 expression. *P < 0.05