Fig. 3

Upregulation of miR-141-3p inhibits EMT in PCa cells. a Gene set enrichment analysis (GSEA) revealed that low expression of miR-141-3p expression significantly and positively correlated with the EMT signatures. b Real-time PCR analysis of miR-141-3p expression inVCaP and C4-2B cells transduced with miR-141-3p or transfected with anti-miR-141-3p compared to controls. Transcript levels were normalized by U6 expression. Error bars represent the mean ± s.d. of three independent experiments. *P < 0.05. c Overexpression of miR-141-3p increased E-cadherin expression and decreased Vimentin and Fibronectin expression in PCa cells; while silencing miR-141-3p decreased E-cadherin expression and increased Vimentin and Fibronectin expression in VCaP and C4-2B cells. α-Tubulin served as the loading control. d Upregulating miR-141-3p converted a stick-like or long spindleshaped mesenchymal profile to a cobblestone-like or a short spindle-shaped epithelial morphology in PC-3 cells. e Upregulating miR-141-3p converted a stick-like or long spindleshaped mesenchymal profile to a cobblestone-like or a short spindle-shaped epithelial morphology in VCaP and C4-2B cells treated with TGF-β (5 ng/ml for 72 h)