Fig. 5

miR-141-3p targets TRAF5 and TRAF6. a Predictive target genes of miR-141-3p from TargetScan, miRanda and miRDB. b Western blotting of TRAF1, TRAF5 and TRAF6 expression in the indicated cells. α-Tubulin served as the loading control. c Predicted miR-141-3p targeting sequence and mutant sequences in 3’UTR s of TRAF5 and TRAF6. d-f Luciferase assay of cells transfected with pmirGLO-3’UTR reporter of TRAF5 and TRAF6 in the miR-141-3p overexpressing and silencing PCa cells. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05. g-i MiRNP IP assay showing the association between miR-141-3p and TRAF5 and TRAF61transcripts in PCa cells cells. Pulldown of IgG antibody served as the negative control. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05