Fig. 6

miR-141-3p inhibits NF-κB signaling pathway. a and b NF-κB transcriptional activity was assessed by luciferase reporter constructs in the indicated cells. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05. c and d Western blotting of nuclear NF-κB/p65 expression in the indicated cells. The nuclear protein p84 was used as the nuclear protein marker. e-g Real-time PCR analysis of Vimentin, TWIST1, SNAIL2, MMP13 and IL11 in the indicated cells. Transcript levels were normalized to U6 expression. Error bars represent the mean ± S.D. of three independent experiments. *P < 0.05