Fig. 3

FOXA1 regulates the expression of PIK3R1. Based on the FOXA1 conserved motif (b), we explored FOXA1 target genes in Cistrome dataset_browser and ChIPBase_v2.0 datasets (a) combined with GO enrichment analysis (c, d), and we selected our target gene of interest, PIK3R1. qPCR revealed that downregulation of FOXA1 decreased the PIK3R1 mRNA level, whereas plasmid overexpression of FOXA1 increased PIK3R1 expression (e, f). In HepG2 (g, h) and Hep3B (i, j) cells, decreased FOXA1 expression promoted PI3Kp85 expression, thereby increasing PI3K/AKT activity, whereas increased FOXA1 expression suppressed PI3K/AKT activity through PI3Kp85 suppression. GAPDH was used as the loading control