Fig. 5

Inhibition of PI3Kp85 reduces cell mobility. (a, b) Transwell migration and invasion assays show that simultaneous knockdown of FOXA1 and PI3Kp85 decreased cell migration and invasion, whereas pAKT levels were decreased compared with knockdown of only FOXA1 (c); GAPDH was used as the loading control. In male HCC tissue, the FOXA1 level was much lower than that in matched non-tumor tissue, whereas PI3Kp85 was highly elevated in HCC tissue (d, e). In stage II male HCC patients, FOXA1 was negatively correlated with PI3Kp85 (f), and low expression of PI3Kp85 predicted a favorable outcome (g) (transwell and IHC magnification ×200)