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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Synergistic antitumor interaction between valproic acid, capecitabine and radiotherapy in colorectal cancer: critical role of p53

Fig. 4

Pro-apoptotic effect induced by VPA/5′-DFUR combination plus 2 Gy RT in CRC cell lines. HT29 and SW620 cells were treated or untreated with VPA 1 mM and 5′-DFUR at the indicated concentration, corresponding to IC30 at 96 h for 24 h followed or not by 2 Gy RT. a Apoptotic effect on HT29 and SW620 cells was evaluated by flow cytometry analysis upon Annexin V-FITC staining after treatment with or without VPA 1 mM and/or 5′-DFUR at the indicated concentration, corresponding to IC15 at 96 h for 24 h and then with or without 2 Gy RT for 48 (HT29) and 24 (SW620) hours. b Cell cycle analysis was performed in HT29 and SW620 cell lines. The percentage of sub G1, G1, S and G2/M population were analyzed by flow cytometry on cells treated for 24 h with or without VPA 1 mM and 5′-DFUR at the indicated concentrations, corresponding to IC15 at 96 h, followed or not by 24 h exposure to 2 Gy RT

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