Fig. 5
Effect of triple combination on the expression and activity of crucial proteins in DNA damage/repair pathways. HT29 and SW620 cells were treated or untreated with VPA 1 mM and 5′-DFUR at the indicated concentrations, corresponding to IC30 at 96 h for 24 h followed or not by 2 Gy RT. TP, TS, γH2AX, phospho-ATM, ATM, phosphop53, p53, BAX, VDAC-1, and AcH3 protein expression were evaluated by western blot analysis 48 h after RT. γ-tubulin was used as protein loading control