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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Silencing of miR-193a-5p increases the chemosensitivity of prostate cancer cells to docetaxel

Fig. 2

miR-193a-5p upregulation suppresses PC3 cell apoptosis. a Western blotting detected PCNA and cleaved caspase-3 proteins in PC3 cells treated with or without H2O2 (100 μM, 12 h). Right panel shows densitometric analysis of three independent experiments. **P < 0.01 vs. vehicle control. b qRT-PCR detected the expression of miR-193a-5p in PC3 cells treated with or without H2O2. *P < 0.05 vs. vehicle control. c Western blotting detected PCNA and cleaved caspase-3 in PC3 cells transfected with miR-193a-5p mimic or control (Ctl) mimic, and then treated with H2O2. Bottom panel shows densitometric analysis. ***P < 0.001 vs. Ctl mimic. d PCNA and cleaved caspase-3 were determined by western blotting in PC3 cells transfected with anti-miR-193a-5p or anti-miR-Ctl, and then treated with H2O2. Bottom panel shows densitometric analysis. **P < 0.01 vs. anti-miR-Ctl. e The TUNEL staining detected H2O2–induced apoptosis in PC3 cells transfected with miR-193a-5p mimic or anti-miR-193a-5p. Blue staining represents the nucleus, and red staining indicates TUNEL-positive cells. Right panel shows the number of TUNEL-positive cells of three independent experiments. Scale bar = 100 μm. *P < 0.05, **P < 0.01 vs. their corresponding control

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