Fig. 5

Bach2 is a direct target of miR-193a-5p. a Prediction of miR-193a-5p binding site at Bach2 3′-UTR. Red color indicates the sequence of the mutated miR-193a-5p binding site. b Luciferase reporter assays were performed in PC3 cells co-transfected cells with miR-193a-5p mimic and wild-type (WT) or mutant (mut) Bach2 3′-UTR-luciferase reporter **P < 0.01 vs. Ctl mimic. c and d PC3 cells were transfected with miR-193a-5p mimic (c) or anti-miR-193a-5p (d), Bach2 and Nrf2 expression was analyzed by Western blotting. Bottom panel shows densitometric analysis of three independent experiments. **P < 0.01, ***P < 0.001 vs. their corresponding control. e Bach2 mRNA was detected by qRT-PCR in BPH (n = 21) and PC tissues (n = 21). *P < 0.01 vs. BPH. f Pearson correlation was used to analyze the relationships between miR-193a-5p and Bach2. (R = −0.4902, P = 0.0282)