Fig. 6

Silencing of miR-193a-5p enhances sensitization of PC3 cells to docetaxel-induced apoptosis through upregulating Bach2 expression. a PC3 cells were co-transfected with HO-1 promoter reporter construct and si-Bach2, and then treated with or without Doc. Luciferase reporter assays were performed. *P < 0.01 vs. empty vector or si-Ctl. b ChIP-qPCR detected Bach2 binding to the HO-1 promoter in PC3 cells transfected with anti-miR-193a-5p or anti-miR-Ctl, and then treated with or without Doc. *P < 0.05 vs. anti-miR-Ctl plus PBS. c Agarose gel electrophoresis for PCR products of chromatin immunoprecipitation showing Bach2 binding to the HO-1 promoter. d PC3 cells were transfected with anti-miR-193a-5p or anti-miR-Ctl, and then treated with or without Doc. Immunofluorescence staining detected the expression of HO-1 (green) and Bach2 (red). Nuclei were staining with DAPI (blue). Scale bars = 75 μm. e PC3 cells were treated as in (d), cell apoptosis was assessed by TUNEL staining. Right panel shows the number of TUNEL-positive cells of three independent experiments. *P < 0.01, **P < 0.01 vs. their corresponding control