Fig. 3
Relevance of the uPAR84–95 sequence to migration, matrigel invasion and trans-endothelial migration of melanoma cells. a Cell migration of A375 cells in Boyden chambers for 4 h at 37 °C toward serum-free medium (CTRL) or medium containing 10% FBS (FBS) in the presence of diluents (None), or 2 μg/mL the indicated antibodies. For quantitative analysis of cell migration, values were reported as percentage of the basal value assessed in the absence of chemoattractant taken as 100% (CTRL). Data are the means ± SD of three independent experiments, performed in triplicate. Statistical significance with ***p < 0.0001. b Matrigel invasion of A375 cells monitored by the xCELLigence system. Cells were seeded on polymerized matrigel and allowed to invade matrigel for 20 h. Lower chambers were filled with serum-free medium (CTRL) or growth medium plus diluents (None) or 2 μg/mL the indicated antibodies. Invasion was monitored in real-time as changes in Cell Index. Data represent mean ± SD from a quadruplicate experiment. c Trans-endothelial migration of A375 cells seeded on an endothelial monolayer in the presence of diluents (None), or 2 μg/mL the indicated antibodies. Data represent mean ± SD from a quadruplicate experiment