Fig. 5

RI-3 inhibits migration and matrigel invasion of A375 melanoma cells. a Wound healing of A375 melanoma in the absence (None) or presence of 10 nM RI-3. Cells were kept in growth medium at 37 °C in a 5% CO₂ of a Zeiss inverted microscope equipped with a motorized stage. One field which includes the scratched path from each dish was selected and scanned sequentially every 30 min for 24 h. Images were recorded at the indicated times by a video-camera connected with a motorized inverted microscope. Original magnification: 50×. b Square root of the wound area measured at the indicated times. c Matrigel invasion of A375 cells monitored by the xCELLigence system for 20 h in the presence or the absence of 10 nM RI-3. Data represent mean ± SD from a quadruplicate experiment. d Slopes represent the change rate of Cell Indexes generated in a 1–18 h time frame