Fig. 4

Effect of different schedules of osimertinib and T-DM1 association in the acquisition of osimertinib resistance. Following the schedules indicated in a, PC9-T790M cells were plated in 96-well plates at a density of 350 cells/well and treated with osimertinib 30 nM and/or 2.5 μg/ml T-DM1. Cells were scored as resistant when colonies with at least 20 cells were detected (b). Results are representative of two independent experiments