Fig. 3

LXA4 downregulates expression of TGF-β1 via FPRL1. a Both Panc-1 and MIA PaCa-2 cells were transfected with siRNA targeting FPRL1 (siFPRL1) or negative control (siNC), and 48 h later, 400 nM LXA4 or vehicle were used to treat these cells for 48 h. Then TGF-β1 was detected by western blot. b Immunofluorescence was used to detect FPRL1 and TGF-β1 in both cell lines administered as previous description. c TGF-β1 was measured by ELISA in the former two cell lines. *, P < 0.05. All data were obtained from at least three independent experiments