Fig. 4

LXA4 reverses mesenchymal phenotypes of pancreatic cancer cells by inhibiting TGF-β1 autocrine. a Panc-1 and MIA PaCa-2 cells were treated with vehicle, LXA4 (400 nM), anti-TGF-β1 neutralized antibody (1 μg/mL), or combined treated with LXA4 and recombinant human TGF-β1 (rhTGF-β1, 5 ng/mL). Then western blotting was used to determine the expression of TGF-β1. b and c The former cells were seeded into matrigel-coated chambers to perform transwell invasion assays and the numbers of passed cells were counted in 10 random fields under 200× magnification at 0th and 24th hour. *, P < 0.05. All data were obtained from at least three independent experiments