Fig. 5
From: Inhibition of EGR1 inhibits glioma proliferation by targeting CCND1 promoter
Overexpression of EGR1 induced by EGF improve proliferation of glioma cells. a Real-time quantitative PCR for EGR1 and CCND1 mRNA expression in U251 cells and U251SLCs after adding EGF. β-ACTIN was used as the loading control. b Immunoblots for EGR1 and CCND1 mRNA expression in U251 cells and U251SLCs after adding EGF. β-ACTIN was used as the loading control. c EdU assay for the proliferation of U251 cells group and U251SLCs group with/without EGF. *P < 0.05, **P < 0.01, ***P < 0.001 (mean ± SEM)