Table 1 Methods for ALT diagnosis in cell cultures and tumor specimens
From: Diagnosis and treatment of ALT tumors: is Trabectedin a new therapeutic option?
| Â | Cell culture | Tumor specimens |
|---|---|---|
Telomerase activity | RTQ-TRAP | RTQ-TRAP |
Telomere’s lenght | Southern blot Q-FISH | Q-FISH |
c-circles quantification | c-circle assay | c-circle assay |
TERRA detection | Northern blot RNA Fish | RNA Fish |
ATRX/DAXX expression | Western blot Immunohistochemistry RTQ-PCR Microarray Deep sequencing | Immunohistochemistry RTQ-PCR Microarray Deep sequencing |
APB detection | Co-IF FISH | Co-IF FISH |