Fig. 1
From: Interaction of the epigenetic integrator UHRF1 with the MYST domain of TIP60 inside the cell
TIP60 interacts with UHRF1 and DNMT1 in HeLa cells. a Schematic diagram of TIP60 wild type tagged with eGFP (i) and UHRF1 tagged with mCherry (ii) at their C-terminus. b Transfection of TIP60-eGFP and UHRF1-mCherry in the nucleus of HeLa cells. White bar indicates size of 5 μm. c Immunoprecipitation of UHRF1-mCherry with anti-mCherry antibody co-immunoprecipitating exogenous TIP60-eGFP and endogenous TIP60. d Reciprocal immunoprecipitation of TIP60-eGFP with anti-eGFP antibody co-immunoprecipitating exogenous UHRF1-mCherry and endogenous UHRF1. e DNMT1 co-immunoprecipitate with UHRF1 and TIP60-eGFP using anti-UHRF1 and anti-eGFP antibody respectively