Fig. 3
From: Interaction of the epigenetic integrator UHRF1 with the MYST domain of TIP60 inside the cell
Interaction between TIP60-eGFP and UHRF1-mCherry in S phase of cell cycle. a-c TIP60-eGFP interaction with UHRF1-mCherry in early, mid and late S phases of cell cycle, respectively. Confocal images of cells labeled with TIP60-eGFP, UHRF1-mCherry, EdU-Alexa 647 and merge, respectively (i - iv). The white bar indicates size of 5 μm. 25 μm × 25 μm FLIM images of HeLa cells transfected with TIP60-eGFP (v) or co-transfected with TIP60-eGFP and UHRF1-mCherry (vi) and lifetime distribution curves of the respected cells (vii). Color scale codes for lifetimes ranging from 1.8 ns (red) to 2.8 ns (blue). d Fluorescence lifetime distributions of TIP60-eGFP (
), TIP60-eGFP EdU labeled cells (
), total TIP60-eGFP + UHRF1-mCherry co-transfected cells (
) and co-transfected cells in S-phase of cell cycle (
). Values are means ± SEM from five independent experiments. For statistical analysis, a Student’s t-test was performed (*** P < 0.001)