Fig. 4
From: Interaction of the epigenetic integrator UHRF1 with the MYST domain of TIP60 inside the cell
In vitro pull-down analysis between His-TIP60WT/mutants and GST-UHRF1. a, Diagram showing His tag TIP60 wild type and mutants. b Western blot of in vitro pull-down assay. His tagged TIP60-WT or mutants were immobilized on Ni-NTA beads and incubated with UHRF1-GST. The complex recovered after washing were subjected to SDS PAGE and analyzed by Western blot. c. Western blot images were quantified by Image Studio Lite (Li-Core Biosciences USA) and statistically analyzed by using Student’s t-test. Values are means ± SEM from three independent experiments (* P < 0.05, **P < 0.01)