Fig. 5
From: Interaction of the epigenetic integrator UHRF1 with the MYST domain of TIP60 inside the cell
Interaction between TIP60-eGFP WT/mutants and UHRF1 evidenced by FRET-FLIM. a Schematic diagram of TIP60WT/mutants tagged with eGFP at the C-terminus. b Lifetime distribution of TIP60-eGFP (
), TIP60 WT-eGFP + UHRF1-mCherry co-transfected cells (
), TIP60 ∆CRD-eGFP + UHRF1-mCherry co-transfected cells (
), TIP60 ∆ZnFr-eGFP + UHRF1-mCherry co-transfected cells (
), TIP60 ∆HAT-eGFP + UHRF1-mCherry co-transfected cells (
), TIP60 ∆MYST-eGFP + UHRF1-mCherry co-transfected cells (
). Values are means ± SEM from three to five independent experiments. For statistical analysis, a Student’s t-test was performed (*** P < 0.001)