Fig. 8

TIP60 overexpression down-regulates its epigenetic partners UHRF1 and DNMT1. TIP60-eGFP was overexpressed in HeLa cells and the effect of this transient overexpression was compared to that of the control HeLa cells, HeLa cells with transfecting agent (JetPEI), HeLa cells with transfection of eGFP alone or TIP60ΔMYST-eGFP. a Western blot results showing down-regulation of UHRF1 and DNMT1 in TIP60-eGFP transfected cells. b Analysis of effect of TIP60-eGFP overexpression on UHRF1. c, Analysis of effect of TIP60-eGFP overexpression on DNMT1. Results indicated are from five independent experiments which are analyzed statistically by Student’s t-test (* P < 0.05, ** P < 0.01, *** P < 0.001). d Effect of TIP60-eGFP overexpression on global DNA methylation. DNA was extracted from HeLa cells transfected with TIP60WT-eGFP and mutants and the methylation levels were compared to control HeLa cells. Results are indicated from three independent experiments and were analyzed by one-way ANOVA with post-hoc Tukey test. (* P < 0.05, ** P < 0.01, *** P < 0.001)