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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: RETRACTED ARTICLE: Curcumin synergizes with 5-fluorouracil by impairing AMPK/ULK1-dependent autophagy, AKT activity and enhancing apoptosis in colon cancer cells with tumor growth inhibition in xenograft mice

Fig. 3

Cellular autophagic turnover by combinational treatment of 5-Fu and curcumin. a. Immunofluorescent images of HCT116 and HT29 cells treated with the pre-Cur protocol. Hoechst 33,342 staining (blue) indicates nucleus and Cyto-ID Green staining (green) autophagy status. b. Flow cytometry analysis of autophagy in HCT116 cells pretreated with varied concentrations of curcumin for 24 h and then 20 μM of 5-Fu for 24 h. The percentage value indicates the proportion of autophagy-positive cells, detected as an increase in the number of FITC (FL-1)-labeled cells. c. Western blot analysis of beclin-1, p62 and LC3II/LC3I in HCT116 cells pretreated with varied concentrations of curcumin for 24 h and then 20 μM of 5-Fu for 24 h. *, p < 0.05, and **, p < 0.01, compared to the vehicle (0 μM curcumin) cell group

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