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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: 5-FU resistant EMT-like pancreatic cancer cells are hypersensitive to photochemical internalization of the novel endoglin-targeting immunotoxin CD105-saporin

Fig. 2

5-FU resistant pancreatic cancer cells display increased uptake and accumulation of TPCS2a, and show increased ROS production following PCT. TPCS2a accumulation with a increasing TPCS2a concentration and b increasing incubation time. The graphs show the median TPCS2a fluorescence intensities in live cells and show the average of three and two individual experiments, respectively (Error bars = S.E.). c Representative epi-fluorescence microscopy images of live 5-FU sensitive and 5-FU resistant cells harvested 1, 2 and 4 h after TPCS2a incubation. Co-stain of TPCS2a and Alexa488-Dextran is indicated by yellow fluorescence in the rightmost images. The experiment was performed once but was also verified for the B1Q and the B1L cells (data not shown), confirming the data in (a) and (b). The scale bar is 20 μm. d Relative ROS generation measured 1 h after light exposure, with increasing PCT (TPCS2a + light) illumination doses (30–120 s). The graph shows normalized median DCF fluorescence intensities representative of three individual experiments. e RNA levels (relative quantity) of SOD1 and SOD2 in all cell lines, as measured by RT-qPCR. Error bars represent standard deviation. Statistically significant difference between 5-FU sensitive and 5-FU resistant lines (P < 0.05) is indicated by *. f Western blot analysis showing SOD1 and SOD2 protein expression in untreated cells. The blot is representative from at least two individual experiments

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