Fig. 3From: 5-FU resistant EMT-like pancreatic cancer cells are hypersensitive to photochemical internalization of the novel endoglin-targeting immunotoxin CD105-saporinCo-treatment with BSO increases the cytotoxic effect of PCT, but not to 5-FU. a 5-FU sensitive (Nt, Nw) and b 5-FU resistant cell lines (B1L, B1Q, B1V) were subjected to co-treatment with BSO and PCT for 72 h after light. BSO (100 μM) was added together with TPCS2a the day before, and BSO was added again after wash (total treatment time: 96 h). c All cell lines were subjected to co-treatment with 100 μM BSO and 1 μg/ml 5-FU for 72 h. Reduction in cell viability (%) relative to untreated cells was measured by MTS assay. The experiments were performed at least 3 times, representative data are shown. Error bars represent SD. Statistically significant difference between PCT and PCT + BSO in a) and b) is indicated by **. P < 0.001Back to article page