Fig. 4

Altered expression and localization of the lysosomal marker LAMP1 in the 5-FUR cell lines. a RNA levels (relative quantity) of LAMP1 in all cell lines, as measured by RT-qPCR. Error bars represent standard deviation. Statistically significant difference between 5-FU sensitive and 5-FU resistant lines (P < 0.05) is indicated by *. b All cell lines were subjected to immunoblotting to detect total levels of LAMP1. The experiment was repeated twice (n = 3), representative blots are shown. c All cell lines were subjected to super resolution microscopy (SIM) following LAMP1 and DAPI stain (as described in Methods). The experiment was repeated twice. The white scale bar on the top images is 10 μm, while the scale bar at the lower (enlarged) image is 1 μm