Fig. 7

Increased expression of CD105 and selective binding of CD105-saporin in 5-FU resistant cells. a RNA levels (relative quantity) of CD105/ENG in all cell lines, as measured by RT-qPCR. Error bars represent standard deviation. Statistically significant difference between 5-FU sensitive and 5-FU resistant lines (P < 0.05) is indicated by *. b All cell lines were subjected to immunoblotting to detect total protein levels of CD105. c Flow cytometric analysis was used to detect membranous expression of CD105 in the 5-FU sensitive cell line Panc03.27S–Nt and the 5-FU resistant cell line Panc03.27R–B1V. d and e All cell lines were treated with CD105-saporin or saporin (d) and anti-CD105 antibody alone (e) for 72 h. Reduction in cell viability (%) relative to untreated cells was measured by MTS assay. The viability experiments were repeated at least 3 times, representative data are shown. Error bars represent SD. Statistically significant difference between treatment with CD105-saporin and saporin alone in (d) is indicated by *. P < 0.05