Fig. 1

Notch1 expression in melanoma cells promoted tumor growth in vivo. a Western blot analysis of Notch1 expression in shNotch1 and shCon cells. b Tumor growth curve of shNotch1 and shCon cells in C57 BL/6 mice. The representative three subcutaneous tumor were shown. c Overall survival rates of shNotch1 and shCon cells in C57 BL/6 mice. d The number of lung tumor foci in C57BL/6 mice intravenously inoculated with B16-shNotch1 or B16-shCon cells. The representative one lung tumor was shown. e Western blot analysis of Notch1 expression in Notch1 overexpressing and GFP control cells. f Tumor growth curve of Notch1 and GFP cells in C57 BL/6 mice. The representative three subcutaneous tumor were shown. g Overall survival rates of GFP and Notch1 cells in C57 BL/6 mice. h The number of lung tumor foci in C57BL/6 mice intravenously inoculated with B16-Notch1 or B16-GFP cells. The representative one lung tumor was shown. 8 mice per group were used in this experiment. *, P < 0.05. “ns”, no statistical significance