Fig. 3

Notch1 expression in melanoma cells inhibited IFN-γ-releasing CTL and NK cell infiltration in tumor . Tumor-DLNs isolated from tumor-bearing mice were analyzed for CD3⁺ CD8⁺ T cells, CD49b⁺ NK cells and IFN-γ-releasing immune cells by flow cytometry. a Flow cytometry analysis of CD3⁺ CD8⁺ T lymphocytes in CD45⁺ gate in tumor-DLNs. b Flow cytometry analysis of CD49b⁺ NK cells in CD45⁺ gate in tumor-DLNs. c Flow cytometry analysis of IFN-γ-releasing CTL in CD45⁺ gate in tumor-DLNs. d Flow cytometry analysis of IFN-γ-releasing CD49b⁺ NK cells in CD45⁺ gate in tumor-DLNs. Data was pooled from 3 independent experiments. *, P < 0.05