Fig. 5From: Notch1 signaling in melanoma cells promoted tumor-induced immunosuppression via upregulation of TGF-β1Notch1 expression in tumor modulated immune cell population and phenotype. Tumor-DLNs and tumor tissue isolated from tumor-bearing mice were analyzed for immune cell population and phenotype by flow cytometry. a Flow cytometry analysis of PD-1 expression on CD4+ and CD8+ T lymphocytes in tumor-DLNs. b Flow cytometry analysis of PD-L1 expression on CD45− melanoma cells in tumor. c Flow cytometry analysis of CD11b+Gr-1+ MDSCs on CD45+ gate in tumor-DLNs. d CD8+ T cells to Tregs ratios in tumor-DLNs. e CD4 + Effector cells to Tregs ratios in tumor-DLNs. Data was pooled from 3 independent experiments. *, P < 0.05Back to article page