Fig. 1From: PDGFA/PDGFRα-regulated GOLM1 promotes human glioma progression through activation of AKTGOLM1 expression is elevated in human gliomas. a qRT-PCR to measure relative expression levels of GOLM1 in different grade glioma and non-neoplastic tissue samples. b Representative images of IHC staining for GOLM1 in human glioma and non-neoplastic brain tissue samples. Scale bar = 200 μm; c Graphic representation of scoring performed on IHC staining for GOLM1 in glioma and non-neoplastic tissue samples. Bar graphs show the mean ± the standard error of the mean (SEM) for each group. d qRT-PCR (upper) and Western blot analysis (lower) of GOLM1 levels in NHA, U87MG, A172 and U251 cells. e Representative images of immunofluorescence staining performed for GOLM1 (red) in U87MG and U251 cells and visualized under fluorescence microscopy. F-actin was stained with phalloidin (green). Nuclei were stained with DAPI (blue). Scale bar = 50 μm. (**P < 0.01, ***P < 0.001)Back to article page