Fig. 3

Silencing of GOLM1 inhibits invasive growth of U251 cells in vivo. a Kaplan-Meier survival analysis of mice implanted with U251-NC (n = 8) and -sh-GOLM1 (n = 8) cells. The log-rank test was used to calculate P-values, which were <0.05. b Representative H&E images of intracranial tumors derived from U251-NC and -sh-GOLM1 cells. White arrows in zoomed image highlight tumor cells that have invaded to adjacent brain tissues. c Representative images of IF performed on U251-NC and -sh-GOLM1 tumors in mouse brains with TRA-1-85 antibody (green). Nuclei were labeled with DAPI (blue), and images were merged. Scale bar = 200 μm. d Representative IHC images of GOLM1 and Ki-67 expression in xenografts derived from the cells indicated. Scale bar = 200 μm. e Graphic representation of IHC scoring of GOLM1 and Ki-67 expression in brain sections derived from U251-NC and -sh-GOLM1 cells. f Representative images of subcutaneous U251-NC and -sh-GOLM1 xenografts after surgical removal are also shown. g Tumor growth curves in nude mice from the U251-NC and -sh-GOLM1 groups. h Tumor weight from the U251-NC and -sh-GOLM1 groups. Data are presented as the mean ± SEM. (**P < 0.01)