Fig. 5From: PDGFA/PDGFRα-regulated GOLM1 promotes human glioma progression through activation of AKTGOLM1 overexpression promotes U87MG cells’ invasion and proliferation in vitro and in vivo. Overexpression of GOLM1 in U87MG cells was confirmed by a qRT-PCR and b western blot analysis. c EdU assays for U87MG-Lenti-NC and -Lenti-GOLM1 cells. Scale bar = 100 μm. d Graphic representation of ratios of EdU positive U87MG- Lenti-NC and -Lenti-GOLM1 cells. Data are presented as the mean ± SEM. e Cell viability of U87MG-Lenti-NC or -Lenti-GOLM1 cells evaluated in the CCK8 assay. f Representative images of the morphology of U87MG- Lenti-NC and -Lenti-GOLM1 cells under bright field microscopy. Scale bar = 100 μm. g Representative images of Transwell assays performed with U87MG-Lenti-NC and -Lenti-GOLM1 cells after incubation for 24 h. Cells were fixed and stained with crystal violet. Scale bar = 50 μm. h Quantification of invaded and migrated cells in Transwell assays. Data are presented as the mean ± SEM. Scale bar = 50 μm. i Kaplan-Meier survival analysis of mice implanted with U87MG-Lenti-NC (n = 8) and -Lenti-GOLM1 (n = 8) cells. The log-rank test was used to calculate P-values, which were <0.05. j Representative H&E images of intracranial tumors derived from U87MG-Lenti-NC and -Lenti-GOLM1 cells. White arrows in the zoomed image highlight tumor cells that have invaded adjacent brain tissues. k Representative images of subcutaneous U87MG-Lenti-NC and -Lenti-GOLM1 xenografts after surgical removal are also shown. l Tumor growth curves in nude mice from the U87MG-Lenti-NC and -Lenti-GOLM1 groups. m Tumor weight from the U87MG-Lenti-NC and -Lenti-GOLM1 groups. Data are presented as the mean ± SEM. (*P < 0.05, **P < 0.01)Back to article page