Fig. 8

LncRNA AK023391 was involved in the regulation of the PI3K/Akt signaling pathway. a Pathway expression profile analysis of differentially expressed coding genes caused by si-AK23391 in AGS cells. b KEGG enrichment analysis of the 10 most important enriched pathways involved in the regulation of AK23391 in gastric cancer (GC) cells. c Gene ratio and enrichment score analysis revealed that PI3K/Akt and FOXO signaling pathways, respectively, had the most obvious enrichment among the altered pathways induced by AK023391 knockdown. d Cluster analysis of the differentially expressed genes involved in the PI3K/Akt and FOXO signaling pathways, induced by AK023391 knockdown. e-f Western blotting validation of the effects of AK023391 knockdown on the expression of PI3K/Akt, NF-κB, p53, and FOXO3a pathways, and their downstream transcription factors c-myb, cyclinB1/G2, and BCL-6 in HGC 27, AGS, and SGC-7901 cells