Fig. 4

MCM6 augments tumor proliferation and metastatic potential in vivo. a Xenografts in nude mice were established by subcutaneous injection of SMMC7721-shMCM6 and control cells. b and c Representative pictures and external whole-body fluorescence images of intrahepatic and lung metastasis, which were obtained 6 weeks after orthotopic liver injection, respectively. *P < 0.05 based on Student’s t test. Scale bar: 5 mm (top), 100 μm (bottom). d Representative IHC images of MCM6, as well as Ki67 and EMT-associated genes in subcutaneous tumors of mice injected with SMMC7721-shMCM6 and control cells. Scale bars: 50 μm